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Proceedings Paper

New cancer-treatment model of photodynamic therapy combined with a type I topoisomerase inhibitor, CPT-11, against HeLa cell tumors in nude mice used by OPO parametric tunable laser
Author(s): Takato O. Yoshida; Eiji Matsuzawa; Tetsumichi Matsuo; Yukio Koide; Susumu Terakawa; Teruo Yokokura; Toru Hirano
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Paper Abstract

A new cancer-treatment model, photodynamic therapy (PDT) combined with a type I topoisomerase inhibitor, camptothecin derivative (CPT-11), against HeLa cell tumors in BALB/c nude mice has been developed using a wide-band tunable coherent light source operated on optical parametric oscillation (OPO parametric tunable laser). The Photosan-3 PDT and CPT-11 combined therapy was remarkably effective, that is the inhibition rate (I.R.) 40 - 80%, as compared to PDT only in vivo. The analysis of HpD (Photosan-3) and CPT-11 effects on cultured HeLa cells in vitro has been studied by a video-enhanced contrast differential interference contrast microscope (VEC-DIC). Photosan-3 with 600 nm light killed cells by mitochondrial damage within 50 min, but not with 700 nm light. CPT-11 with 700 - 400 nm light killed cells within 50 min after nucleolus damage appeared after around 30 min. The localization of CPT-11 in cells was observed as fluorescence images in the nucleus, particularly the nucleoral area produced clear images using an Argus 100.

Paper Details

Date Published: 1 March 1995
PDF: 5 pages
Proc. SPIE 2371, 5th International Photodynamic Association Biennial Meeting, (1 March 1995); doi: 10.1117/12.203369
Show Author Affiliations
Takato O. Yoshida, Hamamatsu Univ. School of Medicine (Japan)
Eiji Matsuzawa, Hamamatsu Photonics KK (Japan)
Tetsumichi Matsuo, Hamamatsu Univ. School of Medicine (Japan)
Yukio Koide, Hamamatsu Univ. School of Medicine (Japan)
Susumu Terakawa, Hamamatsu Univ. School of Medicine (Japan)
Teruo Yokokura, Yakult Central Institute for Microbiological Research (Japan)
Toru Hirano, Hamamatsu Univ. Photonics KK (Japan)


Published in SPIE Proceedings Vol. 2371:
5th International Photodynamic Association Biennial Meeting
Denis A. Cortese, Editor(s)

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