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Proceedings Paper

Near infrared FRET using wide-field fluorescence lifetime imaging in live animals
Author(s): Lingling Zhao; Ken Abe; Margarida Barroso; Xavier Intes
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Paper Abstract

One of the challenges in anti-cancer drug delivery systems is to quantitatively discriminate non-specific receptorindependent tumor accumulation from receptor-mediated uptake into the tumor cells. To overcome this challenge, we develop a new near infrared fluorescence resonance energy transfer fluorescence lifetime imaging (NIR FRET FLIM) technique with wide-field illumination strategies to validate and characterize cellular uptake in both cancer cells and normal cells with different donor-acceptor ratios in vitro and in vivo. Our results demonstrate that NIR FRET FLIM can quantitatively distinguish receptor-bound from unbound donor in live animals with high sensitivity and high accuracy. Thus, it has a great potential for the quantitative detection of targeted delivery systems for diagnostic and therapeutic use.

Paper Details

Date Published: 18 June 2013
PDF: 5 pages
Proc. SPIE 8801, Novel Biophotonic Techniques and Applications II, 88010A (18 June 2013); doi: 10.1117/12.2033588
Show Author Affiliations
Lingling Zhao, Rensselaer Polytechnic Institute (United States)
Ken Abe, Albany Medical College (United States)
Margarida Barroso, Albany Medical College (United States)
Xavier Intes, Rensselaer Polytechnic Institute (United States)

Published in SPIE Proceedings Vol. 8801:
Novel Biophotonic Techniques and Applications II
Alex Vitkin; Arjen Amelink, Editor(s)

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