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Proceedings Paper

Investigations of hematoporphyrine emission properties
Author(s): Miroslaw Kwasny; Zygmunt Mierczyk; Alfreda Graczyk
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Paper Abstract

In this paper the dimerization constant of pure hematoporphyrine was established using absorption method and then fluorescence quantum yield ((phi) ) was calculated as a function of monomeric fraction ((alpha) ). It was our objective to formulate an equation describing the relationships between (phi) , (alpha) and ratio of monomer to dimer molar absorption coefficients ((epsilon) M/(epsilon) D), at a given wavelength. (phi) was also studied as a function of pH value and polarity. A 3-D stack fluorescence plot was also constructed and the possibilities of practical application of the fluorescence effect to the visualization of areas with absorbed are presented.

Paper Details

Date Published: 1 March 1995
PDF: 7 pages
Proc. SPIE 2203, Laser Technology IV: Applications in Medicine, (1 March 1995); doi: 10.1117/12.203332
Show Author Affiliations
Miroslaw Kwasny, Military Univ. of Technology (Poland)
Zygmunt Mierczyk, Military Univ. of Technology (Poland)
Alfreda Graczyk, Military Univ. of Technology (Poland)


Published in SPIE Proceedings Vol. 2203:
Laser Technology IV: Applications in Medicine
Wieslaw L. Wolinski; Tadeusz Kecik, Editor(s)

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