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Proceedings Paper

Magnetomotive imaging of iron oxide nanoparticles as cellular contrast agents for optical coherence tomography
Author(s): Peter Cimalla; Theresa Werner; Maria Gaertner; Claudia Mueller; Julia Walther; Dierk Wittig; Marius Ader; Mike Karl; Edmund Koch
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Paper Abstract

Recent studies in animal models provided proof-of-principle evidence for cell transplantation as a potential future therapeutic approach for retinal pathologies in humans such as Retinitis pigmentosa or age-related macular degeneration. In this case, donor cells are injected into the eye in order to protect or replace degenerating photoreceptors or retinal pigment epithelium. However, currently there is no three-dimensional imaging technique available that allows tracking of cell migration and integration into the host tissue under in vivo conditions. Therefore, we investigate about magnetomotive optical coherence tomography (OCT) of substances labeled with iron oxide nanoparticles as a potential method for noninvasive, three-dimensional cell tracking in the retina. We use a self-developed spectral domain OCT system for high-resolution imaging in the 800 nm-wavelength region. A suitable AC magnetic field for magnetomotive imaging was generated using two different setups, which consist of an electrically driven solenoid in combination with a permanent magnet, and a mechanically driven all-permanent magnet configuration. In the sample region the maximum magnetic flux density was 100 mT for both setups, with a field gradient of 9 T/m and 13 T/m for the solenoid and the allpermanent magnet setup, respectively. Magnetomotive OCT imaging was performed in elastic tissue phantoms and single cells labeled with iron oxide nanoparticles. Particle-induced sub-resolution movement of the elastic samples and the single cells could successfully be detected and visualized by means of phase-resolved Doppler OCT analysis. Therefore, this method is a potential technique to enhance image contrast of specific cells in OCT.

Paper Details

Date Published: 18 June 2013
PDF: 7 pages
Proc. SPIE 8802, Optical Coherence Tomography and Coherence Techniques VI, 88020M (18 June 2013); doi: 10.1117/12.2032813
Show Author Affiliations
Peter Cimalla, Universitätsklinikum Carl Gustav Carus Dresden (Germany)
Theresa Werner, Universitätsklinikum Carl Gustav Carus Dresden (Germany)
Maria Gaertner, Universitätsklinikum Carl Gustav Carus Dresden (Germany)
Claudia Mueller, Universitätsklinikum Carl Gustav Carus Dresden (Germany)
Julia Walther, Universitätsklinikum Carl Gustav Carus Dresden (Germany)
Dierk Wittig, Universitätsklinikum Carl Gustav Carus Dresden (Germany)
Marius Ader, DFG-Ctr. for Regenerative Therapies Dresden (Germany)
Mike Karl, DFG-Ctr. for Regenerative Therapies Dresden (Germany)
Deutsches Zentrum für Neurodegenerative Erkrankungen e. V. (Germany)
Edmund Koch, Universitätsklinikum Carl Gustav Carus Dresden (Germany)


Published in SPIE Proceedings Vol. 8802:
Optical Coherence Tomography and Coherence Techniques VI
Brett E. Bouma; Rainer A. Leitgeb, Editor(s)

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