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Proceedings Paper

Improving TCSPC data acquisition from CMOS SPAD arrays
Author(s): Nikola Krstajić; Simon Poland; David Tyndall; Richard Walker; Simao Coelho; David D.-U. Li; Justin Richardson; Simon Ameer-Beg; Robert Henderson
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Paper Abstract

We present a digital architecture for fast acquisition of time correlated single photon counting (TCSPC) timestamps from 32×32 CMOS SPAD array. Custom firmware was written to select 64 pixels out of 1024 available for fast transfer of TCSPC timestamps. Our 64 channel TCSPC is capable of acquiring up to 10 million TCSPC timestamps per second over a USB2 link. We describe the TCSPC camera (Megaframe), camera interface to the PC and the microscope setup. We characterize the Megaframe camera for fluorescence lifetime imaging (FLIM) including instrument response function, time resolution and variability of both across the array. We show a fluorescence lifetime image of a plant specimen (Convallaria majalis) from a custom-built multifocal multiphoton microscope. The image was acquired in 20 seconds (with average timestamp acquisition rate of 4.7 million counts per second).

Paper Details

Date Published: 17 June 2013
PDF: 8 pages
Proc. SPIE 8797, Advanced Microscopy Techniques III, 879709 (17 June 2013); doi: 10.1117/12.2032807
Show Author Affiliations
Nikola Krstajić, The Univ. of Edinburgh (United Kingdom)
Simon Poland, King's College London (United Kingdom)
David Tyndall, Dialog Semiconductor Ltd. (United Kingdom)
Richard Walker, The Univ. of Edinburgh (United Kingdom)
Simao Coelho, King's College London (United Kingdom)
David D.-U. Li, Univ. of Sussex (United Kingdom)
Justin Richardson, Dialog Semiconductor Ltd. (United Kingdom)
Simon Ameer-Beg, King's College London (United Kingdom)
Robert Henderson, The Univ. of Edinburgh (United Kingdom)


Published in SPIE Proceedings Vol. 8797:
Advanced Microscopy Techniques III
Emmanuel Beaurepaire; Peter T. C. So, Editor(s)

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