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Proceedings Paper

Probe-based confocal laser endomicroscopy in head and neck malignancies: early preclinical experience
Author(s): Anna Englhard; Susanne Girschick; Brigitte Mack; Veronika Volgger; Oliver Gires; Christian Conderman; Herbert Stepp; Christian Stephan Betz
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Paper Abstract

Background: Malignancies of the upper aerodigestive tract (UADT) are conventionally diagnosed by white light endoscopy, biopsy and histopathology. Probe-based Confocal Laser Endomicroscopy (pCLE) is a novel non-invasive technique which offers in vivo surface and sub-surface imaging of tissue. It produces pictures of cellular architecture comparable to histology without the need for biopsy. It has already been successfully used in different clinical subspecialties to help in the diagnosis and treatment planning of inflammatory and neoplastic diseases. PCLE needs to be used in combination with specific or non-specific contrast agents. In this study we evaluated the potential use of pCLE in combination with non-specific and specific contrast agents to distinguish between healthy mucosa and invasive carcinoma. Methods: Tissue samples from healthy mucosa and squamous cell carcinoma of the head and neck were taken during surgery. After topical application of three different contrast agents, samples were examined using different pCLE-probes and a Confocal Laser Scanning Microscope (CLSM). Images were then compared to the corresponding histological slides and cryosections. Results: Initial results show that pCLE in combination with fluorophores allows visualization of cellular and structural components. Imaging of different layers was possible using three distinct pCLEprobes. Conclusion: pCLE is a promising non-invasive technique that may be a useful adjunct in the evaluation, diagnosis and treatment planning of head and neck malignancies.

Paper Details

Date Published: 24 June 2013
PDF: 6 pages
Proc. SPIE 8805, Head and Neck Optical Diagnostics, 880506 (24 June 2013); doi: 10.1117/12.2032628
Show Author Affiliations
Anna Englhard, Klinikum der Univ. München (Germany)
Susanne Girschick, Laser-Forschungslabor (Germany)
Brigitte Mack, Klinikum der Univ. München (Germany)
Veronika Volgger, Klinikum der Univ. München (Germany)
Oliver Gires, Klinikum der Univ. München (Germany)
Christian Conderman, Univ. of California, Irvine (United States)
Herbert Stepp, Laser-Forschungslabor (Germany)
Christian Stephan Betz, Klinikum der Univ. München (Germany)

Published in SPIE Proceedings Vol. 8805:
Head and Neck Optical Diagnostics
Christian Betz; Brian J. F. Wong, Editor(s)

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