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Proceedings Paper

Relative deformability of red blood cells in sickle cell trait and sickle cell anemia by trapping and dragging
Author(s): Rance Solomon; James Cooper; Gabriel Welker; Elaura Aguilar; Brooke Flanagan; Chelsey Pennycuff; David Scott; Anthony Farone; Mary Farone; Daniel Erenso; Robert Mushi; Maria del Pilar Aguinaga
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Paper Abstract

Genetic mutation of the β-globin gene or inheritance of this mutated gene changes the chemical composition of the oxygen-carrying hemoglobin molecule that could lead to either the heterozygote genotype, resulting in sickle cell trait (SCT), or the homozygote genotype, resulting in sickle cell anemia (SCA). These mutations could affect the reversible elastic deformations of the red blood cells (RBCs) which are vital for biological functions. We have investigated this effect by studying the differences in the deformability of RBCs from blood samples of an individual with SCT and an untreated patient with SCA along with hemoglobin quantitation of each blood sample. Infrared 1064 nm laser trap force along with drag shear force are used to induce deformation in the RBCs. Ultra2-High Performance Liquid Chromatography (UHPLC) is used for the hemoglobin quantitation.

Paper Details

Date Published: 24 June 2013
PDF: 5 pages
Proc. SPIE 8803, Medical Laser Applications and Laser-Tissue Interactions VI, 880307 (24 June 2013); doi: 10.1117/12.2032591
Show Author Affiliations
Rance Solomon, Middle Tennessee State Univ. (United States)
James Cooper, Middle Tennessee State Univ. (United States)
Gabriel Welker, Middle Tennessee State Univ. (United States)
Elaura Aguilar, Middle Tennessee State Univ. (United States)
Brooke Flanagan, Middle Tennessee State Univ. (United States)
Chelsey Pennycuff, Middle Tennessee State Univ. (United States)
David Scott, Middle Tennessee State Univ. (United States)
Anthony Farone, Middle Tennessee State Univ. (United States)
Mary Farone, Middle Tennessee State Univ. (United States)
Daniel Erenso, Middle Tennessee State Univ. (United States)
Robert Mushi, Meharry Medical College (United States)
Maria del Pilar Aguinaga, Meharry Medical College (United States)


Published in SPIE Proceedings Vol. 8803:
Medical Laser Applications and Laser-Tissue Interactions VI
Lothar D. Lilge; Ronald Sroka, Editor(s)

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