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Proceedings Paper

Multispot two-photon imaging of calcium waves dynamics in cardial tissue at 16Hz frame rate
Author(s): C. de Mauro; C. A. Cecchetti; D. Alfieri; G. Borile; A. Urbani; M. Mongillo; F. S. Pavone
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Paper Abstract

We report on the characterization and use of a Multispot Multiphoton Microscope, to investigate calcium dynamics at intracellular level. Time resolution of a few milliseconds, even in full frame images at 512×512 pixels, is achieved, in order to get the most information on the evolution and propagation of ionic calcium waves across adjacent cells in an intact cardiac tissue.

Paper Details

Date Published: 17 June 2013
PDF: 5 pages
Proc. SPIE 8797, Advanced Microscopy Techniques III, 87970L (17 June 2013); doi: 10.1117/12.2032499
Show Author Affiliations
C. de Mauro, Light4Tech Firenze S.r.l. (Italy)
C. A. Cecchetti, Light4Tech Firenze S.r.l. (Italy)
D. Alfieri, Light4Tech Firenze S.r.l. (Italy)
G. Borile, Univ. of Padova (Italy)
Venetian Institute of Molecular Medicine (Italy)
A. Urbani, Univ. of Padova (Italy)
Venetian Institute of Molecular Medicine (Italy)
M. Mongillo, Univ. of Padova (Italy)
Venetian Institute of Molecular Medicine (Italy)
F. S. Pavone, Univ. of Florence (Italy)


Published in SPIE Proceedings Vol. 8797:
Advanced Microscopy Techniques III
Emmanuel Beaurepaire; Peter T. C. So, Editor(s)

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