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Proceedings Paper

High resolution quantitative phase imaging in digital holographic microscopy by modulated object illumination with an electrically focusable lens
Author(s): Björn Kemper; Robin Schubert; Angelika Vollmer
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Paper Abstract

In digital holographic microscopy (DHM), scattering patterns that are induced by coherent laser light affect the resolution for the detection of optical path length changes. We present a simple and efficient approach for the reduction of coherent disturbances in quantitative phase imaging in self-interference DHM that is based on amplitude and phase modulation of the sample illumination. The performance of the method for quantitative phase imaging is characterized and the application for quantitative analysis of living cells is illustrated.

Paper Details

Date Published: 17 June 2013
PDF: 4 pages
Proc. SPIE 8797, Advanced Microscopy Techniques III, 87970A (17 June 2013); doi: 10.1117/12.2032497
Show Author Affiliations
Björn Kemper, Univ. of Muenster (Germany)
Robin Schubert, Univ. of Muenster (Germany)
Angelika Vollmer, Univ. of Muenster (Germany)

Published in SPIE Proceedings Vol. 8797:
Advanced Microscopy Techniques III
Emmanuel Beaurepaire; Peter T. C. So, Editor(s)

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