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Proceedings Paper

Interferometric phase microscopy of red blood cells
Author(s): Liang Xue; Nan Sun; Xian Tang; Yin Wang; Shouyu Wang
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Paper Abstract

Quantitative phase imaging of cells with high accuracy in a completely noninvasive manner is a challenging task. To provide a proper solution to this important need, interferometric phase microscopy is described which relies on the off-axis interferometry, confocal microscopy and high-speed image capture technology. Phase retrieval from the single interferogram is done by algorithms based on the fast Fourier transform, traditional Hilbert transform and two-step Hilbert transform, respectively. Furthermore, a phase aberrations compensation approach is applied to correct the phase distribution of the red blood cells obtained via the three methods mentioned before without the pre-known knowledge for removing the wave front curvature introduced by the microscope objectives, off-axis imaging, etc., which otherwise hinders the phase reconstruction. The improved results reveal the better inner structures of the red blood cells. The development of quantitative phase imaging technique is shedding light on their future directions and applications for basic and clinical research.

Paper Details

Date Published: 19 December 2013
PDF: 8 pages
Proc. SPIE 9045, 2013 International Conference on Optical Instruments and Technology: Optoelectronic Imaging and Processing Technology, 90450V (19 December 2013); doi: 10.1117/12.2032442
Show Author Affiliations
Liang Xue, Shanghai Univ. of Electric Power (China)
Nan Sun, Shanghai Institute of Quality Inspection and Technical Research (China)
Xian Tang, Nanjing Univ. of Science and Technology (China)
Yin Wang, Sun Yet-sen Univ. Cancer Ctr. (China)
Shouyu Wang, Nanjing Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 9045:
2013 International Conference on Optical Instruments and Technology: Optoelectronic Imaging and Processing Technology
Xinggang Lin; Jesse Zheng, Editor(s)

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