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Proceedings Paper

Multiphoton intravital microscopy setup to visualize the mouse mammary gland
Author(s): Javier Adur; Ana M. Herrera Torres; Andrius Masedunskas; Mariana O. Baratti; Andre A. de Thomaz; Vitor B. Pelegati; Hernandes F. Carvalho; Carlos L. Cesar
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Paper Abstract

Recently, light microscopy-based techniques have been extended to live mammalian models leading to the development of a new imaging approach called intravital microscopy (IVM). Although IVM has been introduced at the beginning of the last century, its major advancements have occurred in the last twenty years with the development of non-linear microscopy that has enabled performing deep tissue imaging. IVM has been utilized to address many biological questions in basic research and is now a fundamental tool that provide information on tissues such as morphology, cellular architecture, and metabolic status. IVM has become an indispensable tool in numerous areas. This study presents and describes the practical aspects of IVM necessary to visualize epithelial cells of live mouse mammary gland with multiphoton techniques.

Paper Details

Date Published: 17 June 2013
PDF: 6 pages
Proc. SPIE 8797, Advanced Microscopy Techniques III, 879705 (17 June 2013); doi: 10.1117/12.2032439
Show Author Affiliations
Javier Adur, Univ. Estadual de Campinas (Brazil)
National Univ. of Entre Ríos (Argentina)
Ana M. Herrera Torres, CES Univ. (Colombia)
Univ Estadual de Campinas (Brazil)
Andrius Masedunskas, National Institute of Science and Technology on Photonics Applied to Cell Biology (Brazil)
Mariana O. Baratti, National Institute of Science and Technology on Photonics Applied to Cell Biology (Brazil)
Andre A. de Thomaz, Univ. Estadual de Campinas (Brazil)
Vitor B. Pelegati, National Institute of Science and Technology on Photonics Applied to Cell Biology (Brazil)
Hernandes F. Carvalho, Univ. Estadual de Campinas (Brazil)
National Institute of Science and Technology on Photonics Applied to Cell Biology (Brazil)
Carlos L. Cesar, Univ. Estadual de Campinas (Brazil)
National Institute of Science and Technology on Photonics Applied to Cell Biology (Brazil)


Published in SPIE Proceedings Vol. 8797:
Advanced Microscopy Techniques III
Emmanuel Beaurepaire; Peter T. C. So, Editor(s)

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