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Proceedings Paper

Nanoscopy with focused light
Author(s): Stefan W. Hell
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Paper Abstract

For many decades, it has been accepted that the resolution of a lens-based optical microscope is limited to about d =λ (2 NA) < 200 nm. The discovery in the 1990’s that elementary transitions between the states of a fluorophore can be used to eliminate the limiting role of diffraction has led to lens-based light microscopy concepts with resolution down to the nanometer scale1,2. Currently, all far-field fluorescence nanoscopy (superresolution) concepts that have found wider application share a common enabling element: they modulate the fluorescence capability of adjacent features such that they fluoresce sequentially3,4.

Paper Details

Date Published: 10 June 2013
PDF: 3 pages
Proc. SPIE 8882, ROMOPTO 2012: Tenth Conference on Optics: Micro- to Nanophotonics III, 888203 (10 June 2013); doi: 10.1117/12.2032261
Show Author Affiliations
Stefan W. Hell, Max Planck Institute for Biophysical Chemistry (Germany)


Published in SPIE Proceedings Vol. 8882:
ROMOPTO 2012: Tenth Conference on Optics: Micro- to Nanophotonics III
Valentin I. Vlad, Editor(s)

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