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Proceedings Paper

Highly fluorescent semiconducting polymer dots for single-molecule imaging and biosensing
Author(s): Wei Sun; Jiangbo Yu; Fangmao Ye; Yu Rong; Daniel T. Chiu
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Paper Abstract

This paper describes the preparation of semiconducting polymer dots (Pdots) and their application for single-molecule imaging and biosensing. The Pdots possessed high fluorescence brightness, with a 16 nm Pdot being ~9 times brighter than 13 nm Quantum Dots (Qdots). The surface of Pdots was successfully conjugated with streptavidin, which made Pdots suitable for specific subcellular labeling and targeting. The interior composition of Pdots was also successfully modified, through which the Pdots obtained additional functionalities. We demonstrated the utility of gold nanoparticle embedded Pdots in dual-modality imaging. We also demonstrated that Rhodamine B embedded Pdots were able to function as ratiometric temperature sensor in live-cell imaging mode.

Paper Details

Date Published: 11 September 2013
PDF: 9 pages
Proc. SPIE 8812, Biosensing and Nanomedicine VI, 881205 (11 September 2013); doi: 10.1117/12.2028187
Show Author Affiliations
Wei Sun, Univ. of Washington (United States)
Jiangbo Yu, Univ. of Washington (United States)
Fangmao Ye, Univ. of Washington (United States)
Yu Rong, Univ. of Washington (United States)
Daniel T. Chiu, Univ. of Washington (United States)

Published in SPIE Proceedings Vol. 8812:
Biosensing and Nanomedicine VI
Hooman Mohseni; Massoud H. Agahi; Manijeh Razeghi, Editor(s)

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