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Proceedings Paper

Direct measurement of formation of loops in DNA by a human tumor suppressor protein
Author(s): Amy Migliori; Samuel Kung; Danielle Wang; Douglas E. Smith
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Paper Abstract

In previous work we developed methods using optical tweezers to measure protein-mediated formation of loops in DNA structures that can play an important role in regulating gene expression. We previously applied this method to study two-site restriction endonucleases, which were convenient model systems for studying this phenomenon. Here we report preliminary work in which we have applied this method to study p53, a human tumor suppressor protein, and show that we can measure formation of loops. Previous biophysical evidence for loops comes from relatively limited qualitative studies of fixed complexes by electron microscopy4. Our results provide independent corroboration and future opportunities for more quantitative studies investigating structure and mechanics.

Paper Details

Date Published: 12 September 2013
PDF: 10 pages
Proc. SPIE 8810, Optical Trapping and Optical Micromanipulation X, 881030 (12 September 2013); doi: 10.1117/12.2027153
Show Author Affiliations
Amy Migliori, Univ. of California, San Diego (United States)
Samuel Kung, Univ. of California, San Diego (United States)
Danielle Wang, Univ. of California, San Diego (United States)
Univ. of California, Irvine (United States)
Douglas E. Smith, Univ. of California, San Diego (United States)


Published in SPIE Proceedings Vol. 8810:
Optical Trapping and Optical Micromanipulation X
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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