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Proceedings Paper

Second harmonic generation from tyrosine containing peptides
Author(s): M. N. Nasir; E. Bergmann; E. Benichou; I. Russier-Antoine; N. Lascoux; Ch. Jonin; F. Besson; P. F. Brevet
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Paper Abstract

The Second Harmonic Generation (SHG) response from Tyrosine-containing peptides at the air-water interface is presented. First, the quadratic hyperpolarizability of the aromatic amino acid Tyrosine obtained by Hyper Rayleigh Scattering is reported, demonstrating its potentiality as an endogenous molecular probe for SHG studies. Then, the single Tyrosine antimicrobial peptide Mycosubtilin is monitored at the air-water interface and compared to another peptide, Surfactin, lacking a Tyrosine residue. Adsorption kinetics and polarization analysis of the SHG intensity for the peptide monolayers clearly demonstrate that the SHG response from Mycosubtilin arises from Tyrosine. Besides, it confirms that indeed Tyrosine can be targeted as an endogenous molecular probe.

Paper Details

Date Published: 1 October 2013
PDF: 6 pages
Proc. SPIE 8817, Nanobiosystems: Processing, Characterization, and Applications VI, 88170M (1 October 2013); doi: 10.1117/12.2026866
Show Author Affiliations
M. N. Nasir, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, CNRS, Univ. Claude Bernard Lyon 1 (France)
E. Bergmann, Institut Lumière Matière, CNRS, Univ. Claude Bernard Lyon 1 (France)
E. Benichou, Institut Lumière Matière, CNRS, Univ. Claude Bernard Lyon 1 (France)
I. Russier-Antoine, Institut Lumière Matière, CNRS, Univ. Claude Bernard Lyon 1 (France)
N. Lascoux, Institut Lumière Matière, CNRS, Univ. Claude Bernard Lyon 1 (France)
Ch. Jonin, Institut Lumière Matière, CNRS, Univ. Claude Bernard Lyon 1 (France)
F. Besson, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, CNRS, Univ. Claude Bernard Lyon 1 (France)
P. F. Brevet, Institut Lumière Matière, CNRS, Univ. Claude Bernard Lyon 1 (France)


Published in SPIE Proceedings Vol. 8817:
Nanobiosystems: Processing, Characterization, and Applications VI
Norihisa Kobayashi; Fahima Ouchen; Ileana Rau, Editor(s)

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