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Proceedings Paper

The validation of the Z-Scan technique for the determination of plasma glucose
Author(s): Sarah I. Alves; Elaine A. O. Silva; Simone S. Costa; Denise R. N. Sonego; Maira L. Hallack; Ornela L. Coppini; Fernanda Rowies; Ligia A. Azzalis; Virginia B. C. Junqueira; Edimar C. Pereira; Katya C. Rocha; Fernando L. A. Fonseca
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Paper Abstract

Glucose is the main energy source for the human body. The concentration of blood glucose is regulated by several hormones including both antagonists: insulin and glucagon. The quantification of glucose in the blood is used for diagnosing metabolic disorders of carbohydrates, such as diabetes, idiopathic hypoglycemia and pancreatic diseases. Currently, the methodology used for this determination is the enzymatic colorimetric with spectrophotometric. This study aimed to validate the use of measurements of nonlinear optical properties of plasma glucose via the Z-Scan technique. For this we used samples of calibrator patterns that simulate commercial samples of patients (ELITech ©). Besides calibrators, serum glucose levels within acceptable reference values (normal control serum - Brazilian Society of Clinical Pathology and Laboratory Medicine) and also overestimated (pathological control serum - Brazilian Society of Clinical Pathology and Laboratory Medicine) were used in the methodology proposal. Calibrator dilutions were performed and determined by the Z-Scan technique for the preparation of calibration curve. In conclusion, Z-Scan method can be used to determinate glucose levels in biological samples with enzymatic colorimetric reaction and also to apply the same quality control parameters used in biochemistry clinical.

Paper Details

Date Published: 18 November 2013
PDF: 6 pages
Proc. SPIE 8785, 8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications, 87851B (18 November 2013); doi: 10.1117/12.2025994
Show Author Affiliations
Sarah I. Alves, Univ. Federal de São Paulo (Brazil)
Elaine A. O. Silva, Univ. Federal de São Paulo (Brazil)
Fundação do ABC (Brazil)
Simone S. Costa, Fundação do ABC (Brazil)
Denise R. N. Sonego, Univ. Federal de São Paulo (Brazil)
Maira L. Hallack, Univ. Federal de São Paulo (Brazil)
Ornela L. Coppini, Univ. Federal de São Paulo (Brazil)
Fernanda Rowies, Univ. Federal de São Paulo (Brazil)
Ligia A. Azzalis, Univ. Federal de São Paulo (Brazil)
Virginia B. C. Junqueira, Univ. Federal de São Paulo (Brazil)
Edimar C. Pereira, Univ. Federal de São Paulo (Brazil)
Katya C. Rocha, Univ. Federal de São Paulo (Brazil)
Fernando L. A. Fonseca, Univ. Federal de São Paulo (Brazil)
Fundação do ABC (Brazil)


Published in SPIE Proceedings Vol. 8785:
8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications
Manuel Filipe P. C. Martins Costa, Editor(s)

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