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Proceedings Paper

Cryogenic localization of single molecules with angstrom precision
Author(s): Siegfried Weisenburger; Bo Jing; Alois Renn; Vahid Sandoghdar
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Paper Abstract

The precision in localizing a molecule is ultimately determined by the number of detected photons, which is in turn limited by photobleaching. Currently, fluorophores can be routinely localized to a few tens of nanometers at room temperature. In this work we demonstrate localization precision better than 3 Angstrom by substantial improvement of the molecular photostability at cryogenic temperatures. We discuss the challenges, solutions and promise of our methodology for high-performance co-localization and super-resolution microscopy.

Paper Details

Date Published: 20 September 2013
PDF: 9 pages
Proc. SPIE 8815, Nanoimaging and Nanospectroscopy, 88150D (20 September 2013); doi: 10.1117/12.2025373
Show Author Affiliations
Siegfried Weisenburger, Max Planck Institute for the Science of Light (Germany)
Bo Jing, ETH Zurich (Switzerland)
Alois Renn, ETH Zurich (Switzerland)
Vahid Sandoghdar, Max Planck Institute for the Science of Light (Germany)

Published in SPIE Proceedings Vol. 8815:
Nanoimaging and Nanospectroscopy
Prabhat Verma; Alexander Egner, Editor(s)

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