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Proceedings Paper

Far-red/near-infrared fluorescent bioprobes based on biocompatible nanoparticles with aggregation-induced emission characteristics for bioimaging applications
Author(s): Wei Qin; Dan Ding; Bin Liu; Ben Zhong Tang
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Paper Abstract

Light emission of 2-(2,6-bis((E)-4-(diphenylamino)styryl]-4H-pyran-4-ylidene}malononitrile (TPA-DCM) is weakened by aggregate formation. Attaching tetraphenylethene (TPE) units as terminals to TPA-DCM dramatically changes its emission behavior: the resulting fluorogen 2-(2,6-bis((E)-4-(phenyl(4’-(1,2,2-triphenylvinyl)-[1,1’-biphenyl]-4- yl)amino)styryl)-4H-pyran-4-ylidene)malononitrile (TPE-TPA-DCM) is more emissive in the aggregate state, showing a novel phenomenon of aggregation-induced emission (AIE). Formulation of TPE-TPA-DCM using bovine serum albumin (BSA) as the polymer matrix yields uniformly sized protein nanoparticles (NPs) with high brightness and low cytotoxicity. Applications of the fluorogen-loaded BSA NPs for in vitro and in vivo far-red/near-infrared (FR/NIR) bioimaging are successfully demonstrated using MCF-7 breast cancer cells and a murine hepatoma-22 (H22) tumorbearing mice model, respectively. The AIE-active fluorogen-loaded BSA NPs show excellent cancer cell uptake and prominent tumor targeting ability in vivo due to the enhanced permeability and retention effect.

Paper Details

Date Published: 18 September 2013
PDF: 8 pages
Proc. SPIE 8831, Organic Field-Effect Transistors XII; and Organic Semiconductors in Sensors and Bioelectronics VI, 88311H (18 September 2013); doi: 10.1117/12.2023312
Show Author Affiliations
Wei Qin, The Hong Kong Univ. of Science and Technology (China)
Dan Ding, National Univ. of Singapore (Singapore)
Bin Liu, National Univ. of Singapore (Singapore)
Ben Zhong Tang, The Hong Kong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 8831:
Organic Field-Effect Transistors XII; and Organic Semiconductors in Sensors and Bioelectronics VI
Zhenan Bao; Ruth Shinar; Ioannis Kymissis; Iain McCulloch, Editor(s)

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