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Proceedings Paper

Speckle based configuration for simultaneous in vitro inspection of mechanical contractions of cardiac myocyte cells
Author(s): Mark Golberg; Dror Fixler; Asher Shainberg; Sharon Zlochiver; Vicente Micó; Javier Garcia; Yevgeny Beiderman; Zeev Zalevsky
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Paper Abstract

In this manuscript we propose optical lensless configuration for a remote non-contact measuring of mechanical contractions of vast number of cardiac myocytes. All the myocytes were taken from rats, and the measurements were done in an in vitro mode. The optical method is based on temporal analysis of secondary reflected speckle patterns generated in lensless microscope configuration. The processing involves analyzing the movement and the change in the statistics of the generated secondary speckle patterns that are created on top of the cell culture when it is illuminated by a spot of laser beam. The main advantage of the proposed system is the ability to measure many cells simultaneously (approximately one thousand cells) and to extract the statistical data of their movement at once. The presented experimental results also include investigation the effect of isoproteranol on cells contraction process.

Paper Details

Date Published: 23 May 2013
PDF: 7 pages
Proc. SPIE 8792, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials, 87921E (23 May 2013); doi: 10.1117/12.2021642
Show Author Affiliations
Mark Golberg, Tel Aviv Univ. (Israel)
Dror Fixler, Bar-Ilan Univ. (Israel)
Asher Shainberg, Bar-Ilan Univ. (Israel)
Sharon Zlochiver, Tel-Aviv Univ. (Israel)
Vicente Micó, Univ. de València (Spain)
Javier Garcia, Univ. de València (Spain)
Yevgeny Beiderman, Bar-Ilan Univ. (Israel)
Zeev Zalevsky, Bar-Ilan Univ. (Israel)


Published in SPIE Proceedings Vol. 8792:
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials
Pietro Ferraro; Monika Ritsch-Marte; Simonetta Grilli; David Stifter, Editor(s)

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