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Proceedings Paper

Label-free biochemical characterization of bovine sperm cells using Raman microscopy
Author(s): A. C. De Luca; S. Manago; M. A. Ferrara; L. Sirleto; R. Puglisi ; D. Balduzzi; A. Galli; I. Rendina; P. Ferraro; G. Coppola
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Paper Abstract

Raman spectroscopy is a label-free and non-invasive method that measures the inelastic scattered light from a sample giving insight into the vibration eigenmodes of the excited molecules. For these reasons, Raman spectroscopy has been used as a powerful tool to investigate different biological tissues and living cells. In this paper, we present a Raman spectroscopy-based method for sensitive biochemical characterization of bovine sperm cells. Importantly, by analysing separate Raman spectra from the nucleus, acrosomale vesicle and tail of single sperm cells, we are able to identify characteristic Raman features associated with DNA, protein and lipid molecular vibrations for discriminating among different locations inside the cell with sub-micrometric resolution (∼0.3 μm). We demonstrate that our Raman spectroscopy facilitates spectral assignment and increases detection sensitivity, opening the way for novel bio-imaging platforms.

Paper Details

Date Published: 23 May 2013
PDF: 7 pages
Proc. SPIE 8792, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials, 87920E (23 May 2013); doi: 10.1117/12.2021575
Show Author Affiliations
A. C. De Luca, Istituto di Biochimica delle Proteine, CNR (Italy)
S. Manago, Istituto di Biochimica delle Proteine, CNR (Italy)
M. A. Ferrara, Istituto per la Microelettronica e Microsistemi, CNR (Italy)
L. Sirleto, Istituto per la Microelettronica e Microsistemi, CNR (Italy)
R. Puglisi , Istituto Sperimentale Italiano Lazzaro Spallanzani (Italy)
D. Balduzzi, Istituto Sperimentale Italiano Lazzaro Spallanzani (Italy)
A. Galli, Istituto Sperimentale Italiano Lazzaro Spallanzani (Italy)
I. Rendina, Istituto per la Microelettronica e Microsistemi, CNR (Italy)
P. Ferraro, Istituto Nazionale di Ottica, CNR (Italy)
G. Coppola, Istituto per la Microelettronica e Microsistemi, CNR (Italy)


Published in SPIE Proceedings Vol. 8792:
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials
Pietro Ferraro; Monika Ritsch-Marte; Simonetta Grilli; David Stifter, Editor(s)

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