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Proceedings Paper

Optical assembly of bio-hybrid machines
Author(s): Alvaro Barroso Peña; Shirin Landwerth; Mike Woerdemann; Maike Becker; Armido Studer; Cornelia Denz
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Paper Abstract

The use of biomolecular motors as mechanical actuators for the implementation of microsystems and microrobots prospects exciting applications in the field of nano engineering and biomedicine. Particularly, the nanomotors of prokaryotic bacteria are of particular interest since they enable a highly efficiently motion at viscous low Reynolds number environments. By means of sensory-based reactions or signal transduction mechanisms such as phototaxis, aerotaxis, chemotaxis, and magnetotaxis, such sophisticated motion can be exploited to induce these motile bacteria to execute specific tasks. For instance, these microorganisms can be used to power micro- and nanodevices in bacteria baths through the collective motion of several bacteria or to transport microspheres attached to a few bacteria, to mention few applications. However, the interaction and attachment between a biological specimen and an abiotic object that make up the so-called bio-hybrid machines is difficult to control and established schemes are still limited to reaction in solutions, while the attachment of only one bacterium propelling a specific payload is even a more challenging task. In our contribution we present a method based on holographic optical tweezers which enables the assembly of bio-hybrid machines consisting of only one bacterium and a single micrometer payload particle. To demonstrate the feasibility of our approach we used a wild type strain of rod-shaped Bacillus subtilis as a model bacterium and porous microsized crystals (zeolite L) as a model of abiotic object. We show exemplary results of optically constructed bio-hybrid machines, investigate the use of various biocompatible functionalization of the surface of the crystals and comment on promising applications.

Paper Details

Date Published:
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Proc. SPIE 8792, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials, 879217; doi: 10.1117/12.2021573
Show Author Affiliations
Alvaro Barroso Peña, Institute of Applied Physics, University of Muenster (Germany)
Shirin Landwerth, Institute of Applied Physics, University of Muenster (Germany)
Mike Woerdemann, Institute of Applied Physics, University of Muenster (Germany)
Maike Becker, Westfälische Wilhelms-Univ. Münster (Germany)
Armido Studer, Westfälische Wilhelms-Univ. Münster (Germany)
Cornelia Denz, Westfälische Wilhelms-Univ. Münster (Germany)


Published in SPIE Proceedings Vol. 8792:
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials
Pietro Ferraro; Monika Ritsch-Marte; Simonetta Grilli; David Stifter, Editor(s)

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