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Proceedings Paper

Raman and surface-enhanced Raman scattering (SERS) biosensing
Author(s): M. Procházka
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Paper Abstract

Raman scattering (RS) is a widely used vibrational technique providing highly specific molecular spectral patterns. A severe limitation for the application of this spectroscopic technique lies in the low cross section of RS. Surface-enhanced Raman scattering (SERS) spectroscopy overcomes this problem by 6-11 order of magnitude enhancement compared with the standard RS for molecules in the close vicinity of certain rough metal surfaces. Thus, SERS combines molecular fingerprint specificity with potential single-molecule sensitivity. Due to the recent development of new SERS-active substrates, labeling and derivatization chemistry, as well as new instrumentations, SERS became a very promising tool for many varied applications, including bioanalytical studies and sensing. Both intrinsic and extrinsic SERS biosensing schemes have been employed to detect and identify small molecules, nucleic acids and proteins, and also for cellular and in vivo sensing. This contribution gives an overview of recent developments in SERS for sensing and biosensing considering also limitations, possibilities and prospects of this technique.

Paper Details

Date Published: 3 May 2013
PDF: 9 pages
Proc. SPIE 8774, Optical Sensors 2013, 877409 (3 May 2013); doi: 10.1117/12.2021555
Show Author Affiliations
M. Procházka, Charles Univ. in Prague (Czech Republic)


Published in SPIE Proceedings Vol. 8774:
Optical Sensors 2013
Francesco Baldini; Jiri Homola; Robert A. Lieberman, Editor(s)

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