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Proceedings Paper

Quadriwave lateral shearing interferometry as a quantification tool for microscopy. Application to dry mass determination of living cells, temperature mapping, and vibrational phase imaging
Author(s): Serge Monneret; Pierre Bon; Guillaume Baffou; Pascal Berto; Julien Savatier; Sherazade Aknoun; Hervé Rigneault
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Paper Abstract

A Quadri-Wave Lateral Shearing Interferometer (QWLSI) is an efficient tool for measuring phase gradients of optical beams along two perpendicular directions. Post-processing integration then allows obtaining the complete phase spatial distribution of the beam. By placing a QWLSI on the exit image plane of such a microscope, we are able to measure the complex field spatial distribution in this plane, and then to retrieve the quantitative optical path difference (OPD) of the observed sample. Here, we demonstrate that we can extend the technique to new applications, were different physical phenomena produce a given sample-induced change in the phase of the exit optical beam that modulates the incident wavefront. More precisely, we used direct refractive-induced OPD, thermal-induced OPD, and resonant vibrational-induced OPD to produce phase contrast images of living cells, temperature distribution of complex patterns of nanostructures, and Raman spectra of polystyrene beads, respectively. In the case of refractive-induced OPD of living cells, we also show that the OPD distribution of a living cell can be used to monitor its dry mass during the cell cycle.

Paper Details

Date Published: 23 May 2013
PDF: 11 pages
Proc. SPIE 8792, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials, 879209 (23 May 2013); doi: 10.1117/12.2021331
Show Author Affiliations
Serge Monneret, Institut Fresnel, CNRS, Aix-Marseille Univ. (France)
Pierre Bon, Institut Fresnel, CNRS, Aix-Marseille Univ. (France)
Guillaume Baffou, Institut Fresnel, CNRS, Aix-Marseille Univ. (France)
Pascal Berto, Institut Fresnel, CNRS, Aix-Marseille Univ. (France)
Julien Savatier, Institut Fresnel, CNRS, Aix-Marseille Univ. (France)
Sherazade Aknoun, Institut Fresnel, CNRS, Aix-Marseille Univ. (France)
PHASICS S.A. (France)
Hervé Rigneault, Institut Fresnel, CNRS, Aix-Marseille Univ. (France)


Published in SPIE Proceedings Vol. 8792:
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials
Pietro Ferraro; Monika Ritsch-Marte; Simonetta Grilli; David Stifter, Editor(s)

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