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Proceedings Paper

New method of 3D tracking of in vitro cells by digital holographic microscopy
Author(s): Pasquale Memmolo; Maria Iannone; Maurizio Ventre; Paolo A. Netti; Andrea Finizio; Melania Paturzo; Pietro Ferraro
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Paper Abstract

We propose a new strategy of three-dimensional (3D) tracking of living cells by digital holographic microscopy based on the morphological changes of cells during the migration. The typical strategy adopted in digital holography for the tracking of living cells consists into compute the 3D position dividing the calculation process into two parts: the estimation of the focal plane on the amplitude reconstruction of the digital holograms and the estimation of the transverse coordinates by the phase reconstruction of digital holograms computed at distance equal to the estimated focal plane. We propose to use an approximation of the Tamura coefficient, as image contrast measure, for the estimation of the focal plane and a new morphological operator, that is called minimum boundary filter (MBF), from which we compute the transverse coordinates. A comparison with other 3D tracking methods is accomplished.

Paper Details

Date Published: 23 May 2013
PDF: 7 pages
Proc. SPIE 8792, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials, 879210 (23 May 2013); doi: 10.1117/12.2020566
Show Author Affiliations
Pasquale Memmolo, Istituto Italiano di Tecnologia (Italy)
Istituto Nazionale di Ottica, CNR (Italy)
Maria Iannone, Istituto Italiano di Tecnologia (Italy)
Maurizio Ventre, Univ. degli Studi di Napoli Federico II (Italy)
Paolo A. Netti, Istituto Italiano di Tecnologia (Italy)
Andrea Finizio, Istituto Nazionale di Ottica, CNR (Italy)
Melania Paturzo, Istituto Nazionale di Ottica, CNR (Italy)
Pietro Ferraro, Istituto Nazionale di Ottica, CNR (Italy)


Published in SPIE Proceedings Vol. 8792:
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials
Pietro Ferraro; Monika Ritsch-Marte; Simonetta Grilli; David Stifter, Editor(s)

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