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Proceedings Paper

Genetic diversity of phytophthoRA sojae isolates in Heilongjiang province in China assessed by RAPD and EST-SSR
Author(s): J. J. Wu; P. F. Xu; L. J. Liu; J. S. Wang; W. G. Lin; S. Z. Zhang; L. Wei
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Paper Abstract

Random-amplified polymorphic DNA (RAPD) and EST-SSR markers were used to estimate the genetic relationship among thirty-nine P.sojae isolates from three locations in Heilongjiang Province, and nine isolates from Ohio in America were made as reference strains. 10 of 50 RAPD primers and 5 of 33 EST-SSR were polymorphic across 48 P.sojae isolates. Similarity values among P.sojae isolates were from 49% to 82% based on the RAPD data. The similarities based on EST-SSR markers ranged from 47% to 85%. The genetic diversity revealed by EST-SSR marker analysis was higher than that obtained from RAPD. The similarity matrices for the SSR data and the RAPD data were moderately correlated (r = 0.47). Genetic similarity coefficients were also relatively lower, which demonstrated complicated genetic background within each location. The high similarity values range revealed the ability of RAPD/EST-SSR markers to distinguish even among morphological similar phytophthora.

Paper Details

Date Published: 19 March 2013
PDF: 4 pages
Proc. SPIE 8762, PIAGENG 2013: Intelligent Information, Control, and Communication Technology for Agricultural Engineering, 87620T (19 March 2013); doi: 10.1117/12.2020182
Show Author Affiliations
J. J. Wu, Heilongjiang Academy of Agricultural Sciences (China)
P. F. Xu, Northeast Agricultural Univ. (China)
L. J. Liu, Heilongjiang Academy of Agricultural Sciences (China)
J. S. Wang, Heilongjiang Academy of Agricultural Sciences (China)
W. G. Lin, Heilongjiang Academy of Agricultural Sciences (China)
S. Z. Zhang, Northeast Agricultural Univ. (China)
L. Wei, Heilongjiang Academy of Agricultural Sciences (China)


Published in SPIE Proceedings Vol. 8762:
PIAGENG 2013: Intelligent Information, Control, and Communication Technology for Agricultural Engineering
Honghua Tan, Editor(s)

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