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Proceedings Paper

Preparation of ferrous chelate of hairtail (Trichiurus haumela) protein hydrolysate (Fe(II)-HPH) and its antibacterial activity
Author(s): Huimin Lin; Bin Zhang; Tian Yu; Shanggui Deng; Kui-Ying Yin
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Paper Abstract

The preparation of a ferrous chelate of hairtail (Trichiurus haumela) protein hydrolysate (Fe(II)-HPH) and its antibacterial activity were studied. The optimal conditions of hydrolysis by papain and ferrous chelation were obtained by single-factor experiments and orthogonal test, with the antibacterial activities as the index. In addition, the antibacterial activity of Fe(II)-HPH was evaluated using the Plackett–Burman design. The orthogonal test results showed that Fe(II)-HPH had an antibacterial activity of 98.3% under a temperature of 40 °C at pH 6.5 for an enzymolysis duration of eight hours in the presence of 20,000 U/g of enzyme. The Plackett–Burman design analysis showed that the three most significant factors (P < 0.05) influencing the antibacterial activity of Fe(II)-HPH were pH, the concentration (mg/mL), and presence of magnesium sulfate.

Paper Details

Date Published: 19 March 2013
PDF: 8 pages
Proc. SPIE 8762, PIAGENG 2013: Intelligent Information, Control, and Communication Technology for Agricultural Engineering, 876212 (19 March 2013); doi: 10.1117/12.2019704
Show Author Affiliations
Huimin Lin, Zhejiang Ocean Univ. (China)
Bin Zhang, Zhejiang Ocean Univ. (China)
Tian Yu, Nanjing Agricultural Univ. (China)
Shanggui Deng, Zhejiang Ocean Univ. (China)
Kui-Ying Yin, Nanjing Research Institute (China)


Published in SPIE Proceedings Vol. 8762:
PIAGENG 2013: Intelligent Information, Control, and Communication Technology for Agricultural Engineering
Honghua Tan, Editor(s)

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