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Proceedings Paper

Mechanism of sequential order determination in bio-Raman correlation analysis
Author(s): Sota Takanezawa; Yasushi Sako; Yukihiro Ozaki; Shin-ichi Morita
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Paper Abstract

Using the measured Raman spectra of triolein and cholesteryl linolenate, the contradiction caused in determining the sequential orders in the two-dimensional correlation spectroscopy was exemplified, in which time-profiles of four marker bands A, B, C, and D were modeled so that A→B→C→D. Here ‘A→B’ is such notation that we read as ‘A is occurred before B’ or ‘A earlier than B’. The two-dimensional correlation method gave the result B→C→D→A which was contradictive to the initial setting. We confirmed that the increments of distance between the peak positions of the Gaussian type time-profiles ƒ and g, through a threshold, gave the unexpected switch in the sequential order. On the complex plane based on the synchronous and asynchronous axes, the vector g is identical to the synchronous axis in direction; the vector ƒ is crossed through the asynchronous axis corresponding to the increments of distance between the peak positions of ƒ and g. By the conventional rule of the correlation method, the vector ƒ just crossing the asynchronous axis is not allowed to be in the second quadrant, but the vector ƒ is transformed to the fourth quadrant with 180 degrees shifted. In the situation, the vector ƒ is located in the later position for the standard vector g, that is, ƒ is occurred ‘after’ g. Based on the revealed mechanism for the contradiction, the time series correlation analysis may be extended in a more versatile manner to allow bio-Raman correlation analysis on diverse dynamics of bio-molecules in living cells.

Paper Details

Date Published: 20 May 2013
PDF: 6 pages
Proc. SPIE 8879, Nano-Bio Sensing, Imaging, and Spectroscopy, 887902 (20 May 2013); doi: 10.1117/12.2018723
Show Author Affiliations
Sota Takanezawa, RIKEN (Japan)
Kwansei Gakuin Univ. (Japan)
Yasushi Sako, RIKEN (Japan)
Yukihiro Ozaki, Kwansei Gakuin Univ. (Japan)
Shin-ichi Morita, RIKEN (Japan)


Published in SPIE Proceedings Vol. 8879:
Nano-Bio Sensing, Imaging, and Spectroscopy
Shin Won Kang; Seung-Han Park; Luke P. Lee; Ki-Bong Song; Yo Han Choi, Editor(s)

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