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Proceedings Paper

LUSH-based SPR sensor for the detection of alcohols and pheromone
Author(s): Hui-Chong Lau; Yeon-Kyung Lee; Jae-Young Kwon; Young-Soo Sohn; Jeong Ok Lim
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Paper Abstract

Protein is a widely used sensing substrate in the biosensing technology. In the study conducted here, we used odorant binding protein, LUSH from Drosophila as a biosensing substrate in a miniaturized surface plasmon resonance (SPR) sensor. LUSH contains the specific alcohols binding sites, which mediates the detection of alcohols and pheromone. We first modified the surface of the gold sensor chip using the self assembled monolayer in the chloroform solution. The saturated concentration was determined prior to the detection of alcohols and pheromone at various concentrations. The results showed that the LUSH was saturated at 1000 μg/ml on the gold sensor chip. The detection response of LUSH was significant at higher concentration of alcohols. LUSH detected ethanol at concentration ≥50%; propanol was detected at ≥25% whereas pheromone was detected at ≥1.25 μg/μl. The results provide some fundamental information on the potential use of LUSH-based SPR as a simple and easy protein-based sensor in the near future.

Paper Details

Date Published: 20 May 2013
PDF: 5 pages
Proc. SPIE 8879, Nano-Bio Sensing, Imaging, and Spectroscopy, 88790F (20 May 2013); doi: 10.1117/12.2018709
Show Author Affiliations
Hui-Chong Lau, Kyungpook National Univ. (Korea, Republic of)
Yeon-Kyung Lee, Catholic Univ. of Daegu (Korea, Republic of)
Jae-Young Kwon, Sungkyunkwan Univ. (Korea, Republic of)
Young-Soo Sohn, Sungkyunkwan Univ. (Korea, Republic of)
Jeong Ok Lim, Kyungpook National Univ. (Korea, Republic of)

Published in SPIE Proceedings Vol. 8879:
Nano-Bio Sensing, Imaging, and Spectroscopy
Shin Won Kang; Seung-Han Park; Luke P. Lee; Ki-Bong Song; Yo Han Choi, Editor(s)

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