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Proceedings Paper

Directional immobilization of antibody in a SPR sensor using EDC-activated protein A
Author(s): Yeon Kyung Lee; Jeong-Ok Lim; Young-Soo Sohn
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Paper Abstract

The EDC-activated protein A has been utilized to directionally immobilize anti-IgG in a miniaturized SPR sensor to enhance IgG detection capability. The SPR sensor chips modified by a self-assembled monolayer (SAM), protein A and the EDC-activated protein A as the linkage layer were compared by the SPR sensor. The SAM was formed on the Au (gold) surface sensor chip by immersing it in the SAM solution. The protein A was formed by injecting their solution to the Au chip. Thirdly, for the EDC-activated protein A, chemical procedure was carried out for the reactable surface of the Au chip. Anti-IgG, bovine serum albumin (BSA) and IgG (50 ng/ml, 100 ng/ml, 150 ng/ml) had been sequently injected into the SPR sensor. In results, the signal of the anti-IgG immobilized by the SAM was the largest increment among three linkage layers. However, the SPR sensor chip modified by EDC-activated protein A showed the highest sensitivity to IgG. From these results, we concluded that the SPR sensor using the EDC-activated protein A can be used to detect biomolecules with trace level concentration for early diagnosis of disease.

Paper Details

Date Published: 20 May 2013
PDF: 4 pages
Proc. SPIE 8879, Nano-Bio Sensing, Imaging, and Spectroscopy, 887904 (20 May 2013); doi: 10.1117/12.2017746
Show Author Affiliations
Yeon Kyung Lee, Catholic Univ. of Daegu (Korea, Republic of)
Jeong-Ok Lim, Kyungpook National Univ. School of Medicine (Korea, Republic of)
Young-Soo Sohn, Catholic Univ. of Daegu (Korea, Republic of)

Published in SPIE Proceedings Vol. 8879:
Nano-Bio Sensing, Imaging, and Spectroscopy
Shin Won Kang; Seung-Han Park; Luke P. Lee; Ki-Bong Song; Yo Han Choi, Editor(s)

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