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Proceedings Paper

Studies on the correlation with olfactory dysfunction in a transgenic mice model of Alzheimer's disease
Author(s): Ameer Rasheed; Ji Hye Lee; Yoo-Hun Suh; Cheil Moon
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Paper Abstract

Alzheimer’s disease (AD) is a progressively debilitating neurodegenerative disorder characterized by the presence of proteinaceous deposits in the brain. AD often results in olfactory dysfunction and impaired olfactory perceptual acuity may be a potential biomarker for early diagnosis of AD. Until recently, there is no Alzheimer’s nanoscope or any other high-end microscope developed to be capable of seeing buried feature of AD clearly. Modern neuroimaging techniques are more effective only after the occurrence of cognitive impairment. Therefore, early detection of Alzheimer’s disease is critical in developing effective treatment of AD. H and E (Haematoxyline and Eosin) staining is performed for examining gross morphological changes, while TUNEL (transferase (TdT)-mediated dUTP nick end labeling) staining for monitoring neuronal death in the olfactory epithelium (OE). Furthermore, immunohistochemistry and western blot are performed to examine β-amyloid protein expression. AD model animals were Tg2576 (transgenic mice that overexpress a mutated form of the Aβ precursor protein), and 6 month (before onset of AD symptoms) and 14 month (after onset of AD symptoms) old WT (wild type) and transgenic mice were compared in their olfactory system. We found that in OE of Tg2576 mice, thickness and total number of cells were decreased, while the numbers of TUNEL-positive neurons, caspase-3 activation were significantly increased compared with age-matched WT. Our results demonstrate that the olfactory system may get deteriorated before onset of AD symptoms. Our findings imply that an olfactory biopsy could be served as an early and relatively simple diagnostic tool for potential AD patients.

Paper Details

Date Published: 20 May 2013
PDF: 4 pages
Proc. SPIE 8879, Nano-Bio Sensing, Imaging, and Spectroscopy, 88790U (20 May 2013); doi: 10.1117/12.2017726
Show Author Affiliations
Ameer Rasheed, Daegu Gyeongbuk Institute of Science and Technology (Korea, Republic of)
Ji Hye Lee, Daegu Gyeongbuk Institute of Science and Technology (Korea, Republic of)
Yoo-Hun Suh, Daegu Gyeongbuk Institute of Science and Technology (Korea, Republic of)
Cheil Moon, Daegu Gyeongbuk Institute of Science and Technology (Korea, Republic of)


Published in SPIE Proceedings Vol. 8879:
Nano-Bio Sensing, Imaging, and Spectroscopy
Shin Won Kang; Seung-Han Park; Luke P. Lee; Ki-Bong Song; Yo Han Choi, Editor(s)

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