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Proceedings Paper

Integrated biochip for PCR-based DNA amplification and detection on capacitive biosensors
Author(s): D. Moschou; N. Vourdas; M. K. Filippidou; V. Tsouti; G. Kokkoris; G. Tsekenis; I. Zergioti; S. Chatzandroulis; A. Tserepi
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Paper Abstract

Responding to an increasing demand for LoC devices to perform bioanalytical protocols for disease diagnostics, the development of an integrated LoC device consisting of a μPCR module integrated with resistive microheaters and a biosensor array for disease diagnostics is presented. The LoC is built on a Printed Circuit Board (PCB) platform, implementing both the amplification of DNA samples and DNA detection/identification on-chip. The resistive microheaters for PCR and the wirings for the sensor read-out are fabricated by means of standard PCB technology. The microfluidic network is continuous-flow, designed to perform 30 PCR cycles with heated zones at constant temperatures, and is built onto the PCB utilizing commercial photopatternable polyimide layers. Following DNA amplification, the product is driven in a chamber where a Si-based biosensor array is placed for DNA detection through hybridization. The sensor array is tested for the detection of mutations of the KRAS gene, responsible for colon cancer.

Paper Details

Date Published: 28 May 2013
PDF: 9 pages
Proc. SPIE 8765, Bio-MEMS and Medical Microdevices, 87650L (28 May 2013); doi: 10.1117/12.2017690
Show Author Affiliations
D. Moschou, National Ctr. for Scientific Research Demokritos (Greece)
N. Vourdas, National Ctr. for Scientific Research Demokritos (Greece)
M. K. Filippidou, National Ctr. for Scientific Research Demokritos (Greece)
V. Tsouti, National Ctr. for Scientific Research Demokritos (Greece)
G. Kokkoris, National Ctr. for Scientific Research Demokritos (Greece)
G. Tsekenis, Biomedical Research Foundation, Academy of Athens (Greece)
I. Zergioti, National Technical Univ. of Athens (Greece)
S. Chatzandroulis, National Ctr. for Scientific Research Demokritos (Greece)
A. Tserepi, National Ctr. for Scientific Research Demokritos (Greece)


Published in SPIE Proceedings Vol. 8765:
Bio-MEMS and Medical Microdevices
Angeliki Tserepi; Manuel Delgado-Restituto; Eleni Makarona, Editor(s)

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