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Proceedings Paper

Mouse neuroblastoma cell based model and the effect of epileptic events on calcium oscillations and neural spikes
Author(s): Suhwan Kim; Juyeong Baek; Unsang Jung; Sangwon Lee; Woonggyu Jung; Jeehyun Kim; Shinwon Kang
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Paper Abstract

Recently, Mouse neuroblastoma cells are considered as an attractive model for the study of human neurological and prion diseases, and intensively used as a model system in different areas. Among those areas, differentiation of neuro2a (N2A) cells, receptor mediated ion current, and glutamate induced physiological response are actively investigated. The reason for the interest to mouse neuroblastoma N2A cells is that they have a fast growing rate than other cells in neural origin with a few another advantages. This study evaluated the calcium oscillations and neural spikes recording of mouse neuroblastoma N2A cells in an epileptic condition. Based on our observation of neural spikes in mouse N2A cell with our proposed imaging modality, we report that mouse neuroblastoma N2A cells can be an important model related to epileptic activity studies. It is concluded that the mouse neuroblastoma N2A cells produce the epileptic spikes in vitro in the same way as produced by the neurons or the astrocytes. This evidence advocates the increased and strong level of neurotransmitters release by enhancement in free calcium using the 4-aminopyridine which causes the mouse neuroblastoma N2A cells to produce the epileptic spikes and calcium oscillation.

Paper Details

Date Published: 20 May 2013
PDF: 8 pages
Proc. SPIE 8879, Nano-Bio Sensing, Imaging, and Spectroscopy, 88790T (20 May 2013); doi: 10.1117/12.2017666
Show Author Affiliations
Suhwan Kim, Kyungpook National Univ. (Korea, Republic of)
Juyeong Baek, Kyungpook National Univ. (Korea, Republic of)
Unsang Jung, Kyungpook National Univ. (Korea, Republic of)
Sangwon Lee, Kyungpook National Univ. (Korea, Republic of)
Woonggyu Jung, Ulsan National Insititute of Science and Technology (Korea, Republic of)
Jeehyun Kim, Kyungpook National Univ. (Korea, Republic of)
Shinwon Kang, Kyungpook National Univ. (Korea, Republic of)


Published in SPIE Proceedings Vol. 8879:
Nano-Bio Sensing, Imaging, and Spectroscopy
Shin Won Kang; Seung-Han Park; Luke P. Lee; Ki-Bong Song; Yo Han Choi, Editor(s)

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