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Proceedings Paper

Real-time polarimetric biosensing using macroporous alumina membranes
Author(s): Jesús Álvarez; Laura Sola; Geoff Platt; Marina Cretich; Marcus Swann; Marcella Chiari; Daniel Hill; Juan Martínez-Pastor
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Paper Abstract

We report the first demonstration of real-time biosensing in free standing macroporous alumina membranes. The membranes with their 200 nm diameter pores are ideal candidates for biosensing applications where fast response times for small sample volumes are needed as they allow analytes to flow through the pores close to the bioreceptors immobilized on the pores walls. A bulk refractive index sensitivity of 5.2x10-6 refractive index units was obtained from signal responses to different concentrations of NaCl solutions flowing through the pores. Finally, after functionalizing the alumina pore surfaces with an epoxysilane and then spotting it with β-Lactoglobulin protein, the interactions between the β-lactoglobulin and rabbit anti-β-lactoglobulin, as well as the interaction between the rabbit anti-β-lactoglobulin and a secondary antibody anti-rabbit Immunoglobulin G were monitored in real-time.

Paper Details

Date Published: 28 May 2013
PDF: 6 pages
Proc. SPIE 8765, Bio-MEMS and Medical Microdevices, 87650I (28 May 2013); doi: 10.1117/12.2017432
Show Author Affiliations
Jesús Álvarez, Univ. of Valencia (Spain)
Laura Sola, Istituto di Chimica del Riconoscimento Molecolare, CNR (Italy)
Geoff Platt, Farfield Group Ltd. (United Kingdom)
Marina Cretich, Istituto di Chimica del Riconoscimento Molecolare, CNR (Italy)
Marcus Swann, Farfield Group Ltd. (United Kingdom)
Marcella Chiari, Istituto di Chimica del Riconoscimento Molecolare, CNR (Italy)
Daniel Hill, Univ. of Valencia (Spain)
Juan Martínez-Pastor, Univ. of Valencia (Spain)

Published in SPIE Proceedings Vol. 8765:
Bio-MEMS and Medical Microdevices
Angeliki Tserepi; Manuel Delgado-Restituto; Eleni Makarona, Editor(s)

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