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Proceedings Paper

Tubular optical waveguide particle plasmon resonance biosensor for multiplex real-time and label-free detection
Author(s): Chen-Han Huang; Hsing-Ying Lin; Lai-Kwan Chau
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Paper Abstract

A tubular optical waveguide particle plasmon resonance (TW-PPR) sensor is demonstrated for higher-throughput and sensitive label-free biochemical detections. Compared to other evanescent field absorption sensors, the TW-PPR sensor possesses merits of itself being a microchamber of a defined sample volume, a mechanical support for sensor coatings, and ease of systematic multichannel expansion. The sensor resolution is estimated to be 2.6 × 10−6 RIU in measuring solutions of various refractive indices (RIs). Additionally, the multichannel TW-PPR sensing system can perform independent measurements simultaneously and its limit of detection (LOD) of anti-DNP antibody and streptavidin separately measured by DNP-functionalized and biotin-functionalized TW-PPR microchambers is demonstrated to be 1.21 × 10−10 and 2.27 × 10−10 g/ml, respectively. Accurate determinations of these molecules with known concentrations spiked in artificial urine are examined and the sensor responses give excellent correlation with results demonstrated in standard buffer examinations, supporting the utility of the device for analyte screening in more complex media. The TWPPR sensor can be inexpensively fabricated and has a special niche as high-sensitivity refractive index sensor as well as biosensor for label-free monitoring biomolecular interactions in real-time. It is ideally suitable for disposable uses, especially promising for convenient higher-throughput biochemical sensing applications.

Paper Details

Date Published: 3 May 2013
PDF: 7 pages
Proc. SPIE 8774, Optical Sensors 2013, 87741K (3 May 2013); doi: 10.1117/12.2017332
Show Author Affiliations
Chen-Han Huang, National Chung Cheng Univ. (Taiwan)
Hsing-Ying Lin, National Chung Cheng Univ. (Taiwan)
Lai-Kwan Chau, National Chung Cheng Univ. (Taiwan)


Published in SPIE Proceedings Vol. 8774:
Optical Sensors 2013
Francesco Baldini; Jiri Homola; Robert A. Lieberman, Editor(s)

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