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Proceedings Paper

Microorganisms detection on substrates using QCL spectroscopy
Author(s): Amira C. Padilla-Jiménez; William Ortiz-Rivera; John R. Castro-Suarez; Carlos Ríos-Velázquez; Iris Vázquez-Ayala; Samuel P. Hernández-Rivera
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Paper Abstract

Recent investigations have focused on the improvement of rapid and accurate methods to develop spectroscopic markers of compounds constituting microorganisms that are considered biological threats. Quantum cascade lasers (QCL) systems have revolutionized many areas of research and development in defense and security applications, including his area of research. Infrared spectroscopy detection based on QCL was employed to acquire mid infrared (MIR) spectral signatures of Bacillus thuringiensis (Bt), Escherichia coli (Ec) and Staphylococcus epidermidis (Se), which were used as biological agent simulants of biothreats. The experiments were carried out in reflection mode on various substrates such as cardboard, glass, travel baggage, wood and stainless steel. Chemometrics statistical routines such as principal component analysis (PCA) regression and partial least squares-discriminant analysis (PLS-DA) were applied to the recorded MIR spectra. The results show that the infrared vibrational techniques investigated are useful for classification/detection of the target microorganisms on the types of substrates studied.

Paper Details

Date Published: 29 May 2013
PDF: 12 pages
Proc. SPIE 8710, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIV, 871019 (29 May 2013); doi: 10.1117/12.2016099
Show Author Affiliations
Amira C. Padilla-Jiménez, Univ. of Puerto Rico Mayagüez (United States)
William Ortiz-Rivera, Univ. of Puerto Rico Mayagüez (United States)
John R. Castro-Suarez, Univ. of Puerto Rico Mayagüez (United States)
Carlos Ríos-Velázquez, Univ. of Puerto Rico Mayagüez (United States)
Iris Vázquez-Ayala, Univ. of Puerto Rico Mayagüez (United States)
Samuel P. Hernández-Rivera, Univ. of Puerto Rico Mayagüez (United States)


Published in SPIE Proceedings Vol. 8710:
Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIV
Augustus Way Fountain, Editor(s)

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