Share Email Print
cover

Proceedings Paper

Biodiscovery of aluminum binding peptides
Author(s): Bryn L. Adams; Deborah A. Sarkes; Amethist S. Finch; Margaret M. Hurley; Dimitra Stratis-Cullum
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Cell surface peptide display systems are large and diverse libraries of peptides (7-15 amino acids) which are presented by a display scaffold hosted by a phage (virus), bacteria, or yeast cell. This allows the selfsustaining peptide libraries to be rapidly screened for high affinity binders to a given target of interest, and those binders quickly identified. Peptide display systems have traditionally been utilized in conjunction with organic-based targets, such as protein toxins or carbon nanotubes. However, this technology has been expanded for use with inorganic targets, such as metals, for biofabrication, hybrid material assembly and corrosion prevention. While most current peptide display systems employ viruses to host the display scaffold, we have recently shown that a bacterial host, Escherichia coli, displaying peptides in the ubiquitous, membrane protein scaffold eCPX can also provide specific peptide binders to an organic target. We have, for the first time, extended the use of this bacterial peptide display system for the biodiscovery of aluminum binding 15mer peptides. We will present the process of biopanning with macroscopic inorganic targets, binder enrichment, and binder isolation and discovery.

Paper Details

Date Published: 31 May 2013
PDF: 12 pages
Proc. SPIE 8719, Smart Biomedical and Physiological Sensor Technology X, 871909 (31 May 2013); doi: 10.1117/12.2015936
Show Author Affiliations
Bryn L. Adams, U.S. Army Research Lab. (United States)
Deborah A. Sarkes, U.S. Army Research Lab. (United States)
Amethist S. Finch, U.S. Army Research Lab. (United States)
Margaret M. Hurley, U.S. Army Research Lab. (United States)
Dimitra Stratis-Cullum, U.S. Army Research Lab. (United States)


Published in SPIE Proceedings Vol. 8719:
Smart Biomedical and Physiological Sensor Technology X
Brian M. Cullum; Eric S. McLamore, Editor(s)

© SPIE. Terms of Use
Back to Top