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Proceedings Paper

Effect of low level laser therapy and high intensity laser therapy on endothelial cell proliferation in vitro: preliminary communication
Author(s): Malgorzata Lukowicz; Justyna Szymanska; Krzysztof Goralczyk; Andrzej Zajac; Danuta Rość
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Paper Abstract

Background: The main purpose of this study was to analyze the influence of power intensity and wavelength of Low Level Laser Therapy (LLLT) and HILT (High Intensity Laser Therapy) on endothelial cell proliferation.
Material and methods: The tests were done on human umbilical vein endothelial cells (HUVEC). Cultures were exposed to laser irradiation of 660 nm and 670 nm at different dosages, power output was 10 – 40 mW as well as 820 nm with power 100 mW and 808 nm with power 1500 mW. Energy density was from 0.28 to 11,43 J/cm2. Cell proliferation of a control and tested culture was evaluated with a colorimetric device to detect live cells. The tests were repeated 8 times.
Results: We observed good effects of LLLT on live isolated ECs and no effects in experiments on previous deep-frozen cultures. Also HILT stimulated the proliferation of HUVEC.
Conclusion: Endothelial cells play a key role in vascular homeostasis in humans. We observed the stimulatory effect of LLLT and HILT on proliferation of HUVEC. Many factors influence the proliferation of EC, so is it necessary to continue the experiment with different doses, intensity and cell concentration.

Paper Details

Date Published: 22 January 2013
PDF: 8 pages
Proc. SPIE 8703, Laser Technology 2012: Applications of Lasers, 870303 (22 January 2013); doi: 10.1117/12.2012658
Show Author Affiliations
Malgorzata Lukowicz, Nicolaus Copernicus Univ. Collegium Medicum in Bydgoszcz (Poland)
Justyna Szymanska, Nicolaus Copernicus Univ. Collegium Medicum in Bydgoszcz (Poland)
Krzysztof Goralczyk, Nicolaus Copernicus Univ. Collegium Medicum in Bydgoszcz (Poland)
Andrzej Zajac, Bialostocka Technical Univ. (Poland)
Danuta Rość, Nicolaus Copernicus Univ. Collegium Medicum in Bydgoszcz (Poland)


Published in SPIE Proceedings Vol. 8703:
Laser Technology 2012: Applications of Lasers
Wiesław L Woliński; Zdzisław Jankiewicz; Ryszard S. Romaniuk, Editor(s)

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