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Proceedings Paper

Ultrathin antibody networks for detection of antigens
Author(s): Andreas Hartmann; Daniel Bock; Michael Martin; Stefan Seeger
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Paper Abstract

Most affinity sensor principles demand immobilized receptor molecules. A widely used multiple-step technique for covalent immobilization of proteins is based on a silanization procedure and a crosslinking agent, e.g., glutardialdehyde. In the presented work non- amphophilic Langmuir-Blodgett films based on phthalocy-aninato-polysiloxane derivatives or copolyglutamates are used to immobilize antibodies as monolayers. Due to a photopolymerization process of terminal C-C-double bonds at alkylic side chains of the non- amphophilic substance the stability of the films is improved compared to conventional LB films. Antibodies can be immobilized by a one-step-method subsequent to illumination. Polymethyl-methacrylate (PMMA) fibers are found to be ideal substrates for the antibody transfer. Enzyme-linked immunosorbent assays (ELISA) give quite good specificities and densities of active immobilized immunoglobulin (IgG). For antigen detection these films are combined with compact evanescent wave sensors. Antibodies were immobilized at the surface of a waveguide using the Langmuir-Blodgett (LB) technique. The light source is a diode laser, which results in a small and compact set-up and due to the red excitation wavelength the background fluorescence is reduced.

Paper Details

Date Published: 10 February 1995
PDF: 13 pages
Proc. SPIE 2331, Medical Sensors II and Fiber Optic Sensors, (10 February 1995); doi: 10.1117/12.201251
Show Author Affiliations
Andreas Hartmann, Ruprecht-Karls-Univ. Heidelberg (Germany)
Daniel Bock, Ruprecht-Karls-Univ. Heidelberg (Germany)
Michael Martin, Ruprecht-Karls-Univ. Heidelberg (Germany)
Stefan Seeger, Ruprecht-Karls-Univ. Heidelberg (Germany)

Published in SPIE Proceedings Vol. 2331:
Medical Sensors II and Fiber Optic Sensors
Anna Maria Verga Scheggi; Francesco Baldini; Pierre R. Coulet; Otto S. Wolfbeis, Editor(s)

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