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Proceedings Paper

Sensing pads for hybrid and monolithic integrated optical immunosensors
Author(s): Rino E. Kunz; Gert L. Duveneck; Markus Ehrat
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Paper Abstract

Results of theoretical and experimental investigations are reported on the feasibility and the performance of a highly sensitive and stable `standardized' sensing pad suitable for integration into a great variety of miniature hybrid and monolithic integrated optical sensors for biochemical and clinical analysis. The basic type of sensing pad structure consists of sensing layers immobilized at the surface of waveguiding films with a high refractive index deposited on low index substrates. As a simple biochemical model system for the sensing pad development, the binding of immunoglobulin (IgG) to immobilized protein A was chosen. Results obtained for TiO2 waveguides at wavelengths of 633 nm and 785 nm show that these sensing pads feature very high sensitivities and an excellent stability, and that infrared laser diodes are adequate light sources. Resolutions and stabilities in the order of 1/4000 of a monolayer have been demonstrated by performing experiments for such chips in an input grating coupler configuration. Based on these results, surface coverage resolutions in the < 100 fg/mm2 range have been estimated if such sensing pads were used in integrated optical interferometers.

Paper Details

Date Published: 10 February 1995
PDF: 16 pages
Proc. SPIE 2331, Medical Sensors II and Fiber Optic Sensors, (10 February 1995); doi: 10.1117/12.201232
Show Author Affiliations
Rino E. Kunz, Paul Scherrer Institute Zurich (Switzerland)
Gert L. Duveneck, Ciba-Geigy, Corp. (Switzerland)
Markus Ehrat, Ciba-Geigy, Corp. (Switzerland)

Published in SPIE Proceedings Vol. 2331:
Medical Sensors II and Fiber Optic Sensors
Anna Maria Verga Scheggi; Francesco Baldini; Pierre R. Coulet; Otto S. Wolfbeis, Editor(s)

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