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Proceedings Paper

Microscopic imaging of glyceraldehyde-induced tissue glycation with intrinsic second harmonic generation and two-photon fluorescence contrasts
Author(s): Yu Jer Hwang; Joseph Granelli; Manasa Tirumalasetty; Julia Lyubovitsky
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Paper Abstract

The bioinspired approaches to tissue strengthening and preservation rely on non-toxic cross-linking agents one of which is glyceraldehyde. In this study we used multiphoton microscopy that employs second harmonic generation (SHG) contrast to evaluate collagen microstructures and two-photon fluorescence (TPF) contrast to monitor progression of cross-linking upon treatment of tissues with glyceraldehyde. We examined collagen hydrogels assembled at 37 °C and 27 °C, bovine scleral and corneal tissues, skin as well as rat tail tendons. The results show a different effect of glyceraldehyde on collagen microstructures within the above tissues. This effect depends on the original microstructural assembly of collagen within a specific tissue. Our data suggests that epidermis (in skin and cornea) will protect collagen from cross-linking with glyceraldehyde. The work highlights benefits of monitoring progression of collagen cross-linking and effects of cross-linking on fiber microstructures as imaged with SHG and TPF signals.

Paper Details

Date Published: 22 February 2013
PDF: 7 pages
Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 858725 (22 February 2013); doi: 10.1117/12.2012305
Show Author Affiliations
Yu Jer Hwang, Univ. of California, Riverside (United States)
Joseph Granelli, Univ. of California, Riverside (United States)
Manasa Tirumalasetty, Univ. of California, Riverside (United States)
Julia Lyubovitsky, Univ. of California, Riverside (United States)


Published in SPIE Proceedings Vol. 8587:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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