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Proceedings Paper

Raman spectroscopy for bacterial identification and characterization
Author(s): Silvie Bernatová; Ota Samek; Zdeněk Pilát; Mojmír Šerý; Jan Ježek; Vladislav Krzyžánek; Pavel Zemánek; Filip Růžička
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Paper Abstract

The main goal of our investigation is to use Raman tweezers technique so that the responce of Raman scattering on microorganisms suspended in liquid media (bacteria, algae and yeast cells in microfluidic chips) can be used to identify different species. The investigations presented here include identification of different bacteria strains (biofilm-positive and biofilm-negative) and yeast cells by using principal component analysis (PCA). The main driving force behind our investigation was a common problem in the clinical microbiology laboratory - how to distinguish between contaminant and invasive isolates. Invasive bacterial/yeast isolates can be assumed to form a biofilm, while isolates which do not form a biofilm can be treated as contaminant. Thus, the latter do not represent an important virulence factor.

Paper Details

Date Published: 18 December 2012
PDF: 6 pages
Proc. SPIE 8697, 18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 86970Y (18 December 2012); doi: 10.1117/12.2010539
Show Author Affiliations
Silvie Bernatová, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Ota Samek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Zdeněk Pilát, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Mojmír Šerý, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Jan Ježek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Vladislav Krzyžánek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Pavel Zemánek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Filip Růžička, Masaryk Univ. (Czech Republic)


Published in SPIE Proceedings Vol. 8697:
18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics
Jan Peřina; Libor Nozka; Miroslav Hrabovský; Dagmar Senderáková; Waclaw Urbańczyk; Ondrej Haderka; Libor Nožka, Editor(s)

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