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Proceedings Paper

Optimizing DNA staining by Hoechst 33342 for assessment of chromatin organization in living cells
Author(s): Sylvain Paillasson; Michel Robert-Nicoud; Xavier Ronot
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Paper Abstract

In recent years, there has been an increasing interest for applications of fluorescence measurements to studies on many physiological mechanisms in living cells. However, few studies have taken advantage of DNA quantification by fluorometry for dynamic assessment of chromatin organization. This type of approach involves both optimal conditions for DNA staining and the use of several investigation methods such as flow cytometry, image cytometry, laser scanning confocal microscopy and spectral imaging. In this context, this report describes a stoichiometric method for nuclear DNA specific staining, using bisbenzimidazole Hoechst 33342 associated with verapamil, a calcium membrane channel blocker. This method makes it possible to follow variations of nuclear DNA content in cells that are maintained alive.

Paper Details

Date Published: 1 February 1995
PDF: 11 pages
Proc. SPIE 2329, Optical and Imaging Techniques in Biomedicine, (1 February 1995); doi: 10.1117/12.200902
Show Author Affiliations
Sylvain Paillasson, Univ. Joseph Fourier (France)
Michel Robert-Nicoud, Univ. Joseph Fourier (France)
Xavier Ronot, Univ. Joseph Fourier and Univ. Montpellier II (France)


Published in SPIE Proceedings Vol. 2329:
Optical and Imaging Techniques in Biomedicine
Hans-Jochen Foth; Aaron Lewis; Halina Podbielska; Michel Robert-Nicoud; Herbert Schneckenburger; Anthony J. Wilson, Editor(s)

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