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Proceedings Paper

Phosphorescent immunostaining
Author(s): Alexander P. Savitsky; Gely V. Ponomarev; Erkki J. Soini
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Paper Abstract

Phosphorescent metalloporphyrins have evoked interest in the field of bioaffinity assays as sensitive labels because the time-resolved detection of phosphorescence is a very efficient method for background rejection. In this paper, the photoluminescence properties of the two major classes of metalloporphyrins are described, namely Pd- and Pt-porphyrin. Both dyes feature high quantum efficiency, low non-specific adsorption on different surfaces, and sufficient photochemical stability. The measurement of phosphorescence of Pt- and Pd- coproporphyrin labeled compounds from the surface of microparticles can be performed in wet or dry state. The phosphorescence of the label was determined using a microscope equipped with a CCD camera with time-resolved phosphorometric detection principle. Different mounting media, particularly those of non-aqueous mountants forming polymerized matrix, are suitable for phosphorescence microscopy analysis. It is possible to measure the phosphorescence signal in the presence of oxygen.

Paper Details

Date Published: 1 February 1995
PDF: 6 pages
Proc. SPIE 2329, Optical and Imaging Techniques in Biomedicine, (1 February 1995); doi: 10.1117/12.200889
Show Author Affiliations
Alexander P. Savitsky, Institute of Biochemistry (Russia)
Gely V. Ponomarev, Institute of Biochemistry (Russia)
Erkki J. Soini, Univ. of Turku (Finland)


Published in SPIE Proceedings Vol. 2329:
Optical and Imaging Techniques in Biomedicine
Hans-Jochen Foth; Aaron Lewis; Halina Podbielska M.D.; Michel Robert-Nicoud; Herbert Schneckenburger; Anthony J. Wilson, Editor(s)

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