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Proceedings Paper

Fluorescence resonance energy transfer (FRET) microscopy: a tool for in situ study of cellular structures
Author(s): Giovanni F. Bottiroli; Piera Balzarini; Anna Cleta Croce; Donata Locatelli; Simona Vaccino; Carlo Pelliciari
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Paper Abstract

A dye pair characterized by favorable spectral properties allows a simplified analytical procedure, based on the measurements of both donor and acceptor emission in double-stained cytological samples, to be applied to evaluate both the relative efficiency of the energy transfer (FRET) process and its topological distribution. Propidium Iodide, a DNA intercalating agent, has been used in combination with Hoechst 33258, a non-intercalating dye specific for A-T sequences of DNA, to assess the chromatin arrangement in human fibroblasts in both quiescent (G0) and cycling (G1) phases. The results indicate that the cells in the two phases, that cannot be distinguished on the basis of the DNA content, exhibit differences of the FRET efficiency relative value that can be ascribed to chromatin structure modification related to gene activation processes.

Paper Details

Date Published: 1 February 1995
PDF: 8 pages
Proc. SPIE 2329, Optical and Imaging Techniques in Biomedicine, (1 February 1995); doi: 10.1117/12.200887
Show Author Affiliations
Giovanni F. Bottiroli, Univ. di Pavia (Italy)
Piera Balzarini, Univ. di Pavia (Italy)
Anna Cleta Croce, Univ. di Pavia (Italy)
Donata Locatelli, Univ. di Pavia (Italy)
Simona Vaccino, Univ. di Pavia (Italy)
Carlo Pelliciari, Univ. di Pavia (Italy)


Published in SPIE Proceedings Vol. 2329:
Optical and Imaging Techniques in Biomedicine
Hans-Jochen Foth; Aaron Lewis; Halina Podbielska; Michel Robert-Nicoud; Herbert Schneckenburger; Anthony J. Wilson, Editor(s)

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