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Proceedings Paper

Optogenetic mapping of brain circuitry
Author(s): George J. Augustine; Ken Berglund; Harin Gill; Carolin Hoffmann; Malvika Katarya; Jinsook Kim; John Kudolo; Li Ming Lee; Molly Lee; Daniel Lo; Ryuichi Nakajima; Min Yoon Park; Gregory Tan; Yanxia Tang; Peggy Teo; Sachiko Tsuda; Lei Wen; Su-In Yoon
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Paper Abstract

Studies of the brain promise to be revolutionized by new experimental strategies that harness the combined power of optical techniques and genetics. We have mapped the circuitry of the mouse brain by using both optogenetic actuators that control neuronal activity and optogenetic sensors that detect neuronal activity. Using the light-activated cation channel, channelrhodopsin-2, to locally photostimulate neurons allows high-speed mapping of local and long-range circuitry. For example, with this approach we have mapped local circuits in the cerebral cortex, cerebellum and many other brain regions. Using the fluorescent sensor for chloride ions, Clomeleon, allows imaging of the spatial and temporal dimensions of inhibitory circuits in the brain. This approach allows imaging of both conventional "phasic" synaptic inhibition as well as unconventional "tonic" inhibition. The combined use of light to both control and monitor neural activity creates unprecedented opportunities to explore brain function, screen pharmaceutical agents, and potentially to use light to ameliorate psychiatric and neurological disorders.

Paper Details

Date Published: 24 October 2012
PDF: 5 pages
Proc. SPIE 8548, Nanosystems in Engineering and Medicine, 85483Y (24 October 2012); doi: 10.1117/12.2008665
Show Author Affiliations
George J. Augustine, Duke-NUS Medical School (Singapore)
Korea Institute of Science and Technology (Korea, Republic of)
Ken Berglund, Duke Univ. School of Medicine (United States)
Harin Gill, Duke-NUS Medical School (Singapore)
Carolin Hoffmann, Duke-NUS Medical School (Singapore)
Malvika Katarya, Duke-NUS Medical School (Singapore)
Jinsook Kim, Duke-NUS Medical School (Singapore)
John Kudolo, Korea Institute of Science and Technology (Korea, Republic of)
Li Ming Lee, Duke-NUS Medical School (Singapore)
Molly Lee, Korea Institute of Science and Technology (Korea, Republic of)
Daniel Lo, Duke-NUS Medical School (Singapore)
Ryuichi Nakajima, Korea Institute of Science and Technology (Korea, Republic of)
Min Yoon Park, Korea Institute of Science and Technology (Korea, Republic of)
Gregory Tan, Duke-NUS Medical School (Singapore)
Yanxia Tang, Duke-NUS Medical School (Singapore)
Peggy Teo, Duke-NUS Medical School (Singapore)
Sachiko Tsuda, Duke-NUS Medical School (Singapore)
Lei Wen, Korea Institute of Science and Technology (Korea, Republic of)
Su-In Yoon, Duke-NUS Medical School (Singapore)

Published in SPIE Proceedings Vol. 8548:
Nanosystems in Engineering and Medicine
Sang H. Choi; Jin-Ho Choy; Uhn Lee; Vijay K. Varadan, Editor(s)

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