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Proceedings Paper

Microfluidic systems for optical sorting
Author(s): Jan Ježek; Zdeněk Pilát; Mojmír Šerý; Jan Kaňka; Ota Samek; Silva Bernatová; Pavel Zemánek
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Paper Abstract

We have constructed a device for active optical manipulation and Raman spectral analysis in a microfluidic channel for efficient, nondestructive and contactless sorting of biological samples based on the Raman spectroscopic characteristics of living cells. In our previous work, we have linked such Raman spectral characteristics of microalgal lipid bodies with the unsaturation or carotene concentration via a calibration curve. As the sorting platform we have used a combination of fast galvano-optic laser steering system and specially designed microfluidic chips. We used X shaped channels with two input and output ports, and also several differently shaped variants. The steerable trapping laser beam was designed to move the cell to the specified locations and confine the cell for the time period needed for the analysis.

Paper Details

Date Published: 18 December 2012
PDF: 9 pages
Proc. SPIE 8697, 18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 86970W (18 December 2012); doi: 10.1117/12.2008649
Show Author Affiliations
Jan Ježek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Zdeněk Pilát, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Mojmír Šerý, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Jan Kaňka, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Ota Samek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Silva Bernatová, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Pavel Zemánek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)


Published in SPIE Proceedings Vol. 8697:
18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics
Jan Peřina; Libor Nozka; Miroslav Hrabovský; Dagmar Senderáková; Waclaw Urbańczyk; Ondrej Haderka; Libor Nožka, Editor(s)

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