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Proceedings Paper

A portable microfluidic-based biophotonic sensor for extracellular H2O2 measurements
Author(s): V. Koman; G. Suárez; Ch. Santschi; V. J. Cadarso; J. Brugger; N. von Moos; V. I. Slaveykova; O. J. F. Martin
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Paper Abstract

In this work a portable analytical biosensor for real-time extracellular monitoring of released hydrogen peroxide (H2O2 ) is presented. The biosensor is based on the optical detection of the cytochrome c (cyt c) oxidation state. The setup consists of an integrated microscope combined with a compact spectrometer. The light being absorbed by cyt c is enhanced via multiscattering produced by random aggregates of polystyrene beads in a cross-linked cyt c matrix. Using ink-jet printing technique, the sensing elements, namely cyt c loaded polystyrene aggregates, are fabricated with high reliability in terms of repeatability of size and sensitivity. Additionally, the sensing elements are enclosed in a microfluidic channel assuring a fast and efficient analytes delivery. As an example, the effect of trace concentrations of functionalized cadmium selenide/zinc sulfide (CdSe/ZnS) core shell quantum dots on the green algae Chlamydomonas reinhardtii is investigated, showing extracellular H2O2 release with different production rates over a period of 1 hour. In conclusion, the presented portable biosensor enables the highly sensitive and non-invasive real-time monitoring of the cell metabolism of C. reinhardtii.

Paper Details

Date Published: 22 March 2013
PDF: 7 pages
Proc. SPIE 8572, Advanced Biomedical and Clinical Diagnostic Systems XI, 857218 (22 March 2013); doi: 10.1117/12.2008329
Show Author Affiliations
V. Koman, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
G. Suárez, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Ch. Santschi, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
V. J. Cadarso, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
J. Brugger, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
N. von Moos, Institute F. A. Forel, Univ. of Geneva (Switzerland)
V. I. Slaveykova, Institute F. A. Forel, Univ. of Geneva (Switzerland)
O. J. F. Martin, Ecole Polytechnique Fédérale de Lausanne (Switzerland)


Published in SPIE Proceedings Vol. 8572:
Advanced Biomedical and Clinical Diagnostic Systems XI
Anita Mahadevan-Jansen; Tuan Vo-Dinh; Warren S. Grundfest, Editor(s)

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