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Proceedings Paper

Application of speckle techniques to studies of phase separation in eye lens homogenate
Author(s): Bronislaw Grzegorzewski; Marek Syroczynski; Malgorzata Pyskir
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Paper Abstract

The temperature reversible phenomenon called phase separation is one of the mechanisms of cataract formation. At a sufficiently low temperature the protein solution separates into protein-rich and protein-poor domains. When light propagates through the medium the random refractive index fluctuations associated with the domains produce intense scattering. It is shown that a thin layer of eye lens homogenate at a sufficiently low temperature produces speckle pattern. This pattern is polarized, Gaussian and partially developed. The speckle pattern is a result of a single scattering. We have investigated the speckle pattern for the case of an induced phase separation. The specular component of scattered radiation is measured in dependence on the temperature. At 18 degree(s)C the first-order statistical properties of the speckle pattern are investigated. The pattern is investigated in the far-field region. The probability density function of speckle intensity and the average contrast of the speckle intensity is measured. Also some results related to the second order statistics are given. It is assumed that the object is phase modulation type. The standard deviations of phase variations and correlation length of the phase variations are estimated.

Paper Details

Date Published: 31 January 1995
PDF: 9 pages
Proc. SPIE 2326, Photon Transport in Highly Scattering Tissue, (31 January 1995); doi: 10.1117/12.200826
Show Author Affiliations
Bronislaw Grzegorzewski, Academy of Technology and Agriculture and Medical Academy of Bydgoszcz (Poland)
Marek Syroczynski, Medical Academy of Bydgoszcz (Poland)
Malgorzata Pyskir, Medical Academy of Bydgoszcz (Poland)

Published in SPIE Proceedings Vol. 2326:
Photon Transport in Highly Scattering Tissue
Sigrid Avrillier; Britton Chance; Gerhard J. Mueller; Alexander V. Priezzhev; Valery V. Tuchin, Editor(s)

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