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Proceedings Paper

Optical tweezers based measurement of PLGA-NP interaction with prostate cancer cells
Author(s): Thea Blesener; Argha Mondal; Jyothi U. Menon; Kytai T. Nguyen; Samarendra Mohanty
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Paper Abstract

In order to quantify the binding capacities of polymeric, biodegradable and biocompatible poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs), conjugated with either R11 peptides or Folic Acid, the strength by detach from prostate cancer cells (PCCs) was measured via optical tweezers based measurements. Specific nanoparticle drug delivery eliminates the previously used diffuse, full-body application of potent cancer drugs by localizing drug delivery to malignant cells. Precise monitoring of NP position in the trap near the PCC membrane using a fluorescence imaging based method enabled calibration of the trap stiffness and subsequent force measurements. By defining the force with which the many diverse conjugates and coatings of different types of NPs bind the vast array of cancer cell types, chemotherapeutic drugs can be delivered in a specific manner with the optimal particle and corresponding conjugates. Further, and most significantly, the rupture force measurements will reveal whether or not targeted nanoparticles can overcome the force of blood attempting to pull the particle from designated cells. Our preliminary study revealed that the binding between PLGA-NPs and prostate cancer cells is enhanced by coating with folic acid or R11 peptides. These conjugates increase the force required to detach the particle thus allowing particles to overcome drag force of the blood in prostate capillary systems.

Paper Details

Date Published: 19 February 2013
PDF: 7 pages
Proc. SPIE 8594, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications X, 859407 (19 February 2013); doi: 10.1117/12.2008237
Show Author Affiliations
Thea Blesener, The Univ. of Texas at Arlington (United States)
Argha Mondal, The Univ. of Texas at Arlington (United States)
Indian Institute of Science Education and Research Kolkata (India)
Jyothi U. Menon, The Univ. of Texas at Arlington (United States)
Kytai T. Nguyen, The Univ. of Texas at Arlington (United States)
Samarendra Mohanty, The Univ. of Texas at Arlington (United States)


Published in SPIE Proceedings Vol. 8594:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications X
Alexander N. Cartwright; Dan V. Nicolau, Editor(s)

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