Share Email Print
cover

Proceedings Paper

Oxygen microenvironment affects the uptake of nanoparticles in head and neck tumor cells
Author(s): Eunice Y. Chen; Sasson Hodge; Katherine Tai; Huagang Hou; Nadeem Khan; P. Jack Hoopes; Kimberley S. Samkoe
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Survival of head and neck cancer patients has not improved in several decades despite advances in diagnostic and therapeutic techniques. Tumor hypoxia in head and neck cancers is a critical factor that leads to poor prognosis, resistance to radiation and chemotherapies, and increased metastatic potential. Magnetic nanoparticle hyperthermia (mNPHT) is a promising therapy for hypoxic tumors because nanoparticles (NP) can be directly injected into, or targeted to, hypoxic tumor cells and exposed to alternating magnetic fields (AMF) to induce hyperthermia. Magnetic NPHT can improve therapeutic effectiveness by two modes of action: 1) direct killing of hypoxic tumor cells; and 2) increase in tumor oxygenation, which has the potential to make the tumor more susceptible to adjuvant therapies such as radiation and chemotherapy. Prior studies in breast cancer cells demonstrated that a hypoxic microenvironment diminished NP uptake in vitro; however, mNPHT with intratumoral NP injection in hypoxic tumors increased tumor oxygenation and delayed tumor growth. In this study, head and neck squamous cell carcinoma (HNSCC) cell lines were incubated in normoxic, hypoxic, and hyperoxic conditions with iron oxide NP for 4-72 hours. After incubation, the cells were analyzed for iron uptake by mass spectrometry, Prussian blue staining, and electron microscopy. In contrast to breast cancer cells, uptake of NPs was increased in hypoxic microenvironments as compared to normoxic conditions in HNSCC cells. In future studies, we will confirm the effect of the oxygen microenvironment on NP uptake and efficacy of mNPHT both in vitro and in vivo.

Paper Details

Date Published: 26 February 2013
PDF: 9 pages
Proc. SPIE 8584, Energy-based Treatment of Tissue and Assessment VII, 85840F (26 February 2013); doi: 10.1117/12.2008067
Show Author Affiliations
Eunice Y. Chen, Dartmouth-Hitchcock Medical Ctr. (United States)
Geisel School of Medicine (United States)
Sasson Hodge, Dartmouth-Hitchcock Medical Ctr. (United States)
Katherine Tai, Dartmouth-Hitchcock Medical Ctr. (United States)
Huagang Hou, Dartmouth-Hitchcock Medical Ctr. (United States)
Nadeem Khan, Dartmouth-Hitchcock Medical Ctr. (United States)
P. Jack Hoopes, Dartmouth-Hitchcock Medical Ctr. (United States)
Geisel School of Medicine (United States)
Thayer School of Engineering at Dartmouth (United States)
Kimberley S. Samkoe, Dartmouth-Hitchcock Medical Ctr. (United States)
Geisel School of Medicine (United States)
Thayer School of Engineering at Dartmouth (United States)


Published in SPIE Proceedings Vol. 8584:
Energy-based Treatment of Tissue and Assessment VII
Thomas P. Ryan, Editor(s)

© SPIE. Terms of Use
Back to Top